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m51 super 8x fopflash  (Addgene inc)


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    Structured Review

    Addgene inc m51 super 8x fopflash
    M51 Super 8x Fopflash, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m51 super 8x fopflash/product/Addgene inc
    Average 94 stars, based on 245 article reviews
    m51 super 8x fopflash - by Bioz Stars, 2026-03
    94/100 stars

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    Reagents used in this study
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    Addgene inc fopflash
    (A) Genomic interval containing TNIK and RE- TNIK in hg38 coordinates. (B) RE- TNIK is a cis -regulated open chromatin region with increased chromatin accessibility in chimpanzee compared to human NPCs. The location of the three gRNAs used to target RE- TNIK are indicated. (C) 50µM of KY-05009, a TNIK inhibitor, decreased luciferase activity from the Wnt <t>reporter</t> <t>TOPflash</t> in the presence of 50µM of CHIR-99021, a Wnt agonist (red). KY-05009 had no effect on luciferase activity from the control reporter <t>FOPflash</t> (gray). (D) Volcano plot of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. Genes significant at 5% FDR are in red. Highly significant genes, as well as examples of genes associated with Wnt signaling, are labelled. (E) Significant terms from gene-set enrichment analysis of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. See also Fig. S4, Table S4, and Materials and Methods.
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    Reagents used in this study

    Journal: Genes & Development

    Article Title: Fatty acid uptake activates an AXL–CAV1–β-catenin axis to drive melanoma progression

    doi: 10.1101/gad.351985.124

    Figure Lengend Snippet: Reagents used in this study

    Article Snippet: Super8XFOPflash , Obtained from R. Moon , , Addgene_12457.

    Techniques: Software, Transduction, Virus, Subcloning, Bacteria, Recombinant, Bradford Assay, Protease Inhibitor, RNA Extraction, Reporter Assay, Bicinchoninic Acid Protein Assay, Quantitative RT-PCR, Negative Control

    (A) Genomic interval containing TNIK and RE- TNIK in hg38 coordinates. (B) RE- TNIK is a cis -regulated open chromatin region with increased chromatin accessibility in chimpanzee compared to human NPCs. The location of the three gRNAs used to target RE- TNIK are indicated. (C) 50µM of KY-05009, a TNIK inhibitor, decreased luciferase activity from the Wnt reporter TOPflash in the presence of 50µM of CHIR-99021, a Wnt agonist (red). KY-05009 had no effect on luciferase activity from the control reporter FOPflash (gray). (D) Volcano plot of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. Genes significant at 5% FDR are in red. Highly significant genes, as well as examples of genes associated with Wnt signaling, are labelled. (E) Significant terms from gene-set enrichment analysis of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. See also Fig. S4, Table S4, and Materials and Methods.

    Journal: bioRxiv

    Article Title: Human-chimpanzee tetraploid system defines mechanisms of species-specific neural gene regulation

    doi: 10.1101/2025.03.31.646367

    Figure Lengend Snippet: (A) Genomic interval containing TNIK and RE- TNIK in hg38 coordinates. (B) RE- TNIK is a cis -regulated open chromatin region with increased chromatin accessibility in chimpanzee compared to human NPCs. The location of the three gRNAs used to target RE- TNIK are indicated. (C) 50µM of KY-05009, a TNIK inhibitor, decreased luciferase activity from the Wnt reporter TOPflash in the presence of 50µM of CHIR-99021, a Wnt agonist (red). KY-05009 had no effect on luciferase activity from the control reporter FOPflash (gray). (D) Volcano plot of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. Genes significant at 5% FDR are in red. Highly significant genes, as well as examples of genes associated with Wnt signaling, are labelled. (E) Significant terms from gene-set enrichment analysis of differential gene expression between chimpanzee NPCs infected with gRNAs targeting RE- TNIK and NTC gRNAs. See also Fig. S4, Table S4, and Materials and Methods.

    Article Snippet: For the Wnt reporter assays, NPCs were transfected with 0.2µl Lipofectamine Stem Transfection Reagent, 2ng of pNL1.1.TK (Promega, cat #N1501), and 198ng of TOPflash (Addgene, cat #12456) or FOPflash (Addgene, cat #12457) ( ) in 10µl Opti-MEM.

    Techniques: Luciferase, Activity Assay, Control, Gene Expression, Infection